Fast STED microscopy with continuous wave fiber lasers

Fast STED microscopy with continuous wave fiber lasers.

We report on fast beam-scanning stimulated-emission-depletion (STED) microscopy in the visible range using for resolution enhancement compact, low cost and turn-key continuous wave (CW) fiber lasers emitting at 592 nm. Spatial resolutions of 35 to 65 nm in the focal plane are shown for various samples including fluorescent nanoparticles, immuno-stained cells with a non-exhaustive selection of 5...

High-Power Continuous-Wave Directly-Diode-Pumped Fiber Raman Lasers

We describe novel fiber Raman lasers pumped directly by spectrally combined high power multimode laser diodes at ∼975 nm and emitting at ∼1019 nm. With a commercial multimode graded-index fiber, we reached 20 W of laser output power with a record slope efficiency of 80%. With an in-house double-clad fiber, the beam quality improved to M = 1.9, albeit with lower output power and slope efficiency...

Photocoagulation delivery systems for continuous-wave lasers.

Continuous-wave Lasers in Polymer waveguides

Channel waveguides based on a polymer, 6fluorinated-dianhydride/epoxy, which is actively doped with a rare-earth-ion-doped complex, Nd(thenoyltrifluoroacetone)3 1,10-phenanthroline, have been fabricated. Photoluminescence peaks at 880 nm, 1060 nm, and 1330 nm have been experimentally observed. By optimization of the fabrication procedure of both, host material and optical structure, continuous-...

Dissecting tripartite synapses with STED microscopy.

The concept of the tripartite synapse reflects the important role that astrocytic processes are thought to play in the function and regulation of neuronal synapses in the mammalian nervous system. However, many basic aspects regarding the dynamic interplay between pre- and postsynaptic neuronal structures and their astrocytic partners remain to be explored. A major experimental hurdle has been ...